TSC1 anticorps (Middle Region)

N° du produit ABIN6990536

L’anticorps Lapin Polyclonal anti-TSC1 a été validé pour ELISA, WB, IF et ICC. Il convient pour détecter TSC1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour TSC1 anticorps (Middle Region) (ABIN6990536)

Antigène: TSC1 (Tuberous Sclerosis 1 (TSC1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TSC1 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-TSC1 anticorps

Épitope: AA 220-270, Middle Region

Specificité: At least two isoforms of TSC1 are known to exist, this antibody will detect both isoforms. TSC1 antibody is predicted to not cross-react with TSC2.

Purification: TSC1 Antibody is affinity chromatography purified via peptide column.

Immunogène: TSC1 antibody was raised against a 15 amino acid synthetic peptide from the middle region of human TSC1. The immunogen is located within amino acids 220 - 270 of TSC1.

Isotype: IgG

Information d'application

Indications d'application: TSC1 antibody can be used for the detection of TSC1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL. For immunofluorescence start at 2 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: TSC1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: TSC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur TSC1

Antigène: TSC1 (Tuberous Sclerosis 1 (TSC1))

Autre désignation: TSC1

Sujet: TSC1 Antibody: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

Poids moléculaire: Predicted: 128 kDa

Observed: 135 kDa

ID gène: 7248

UniProt: Q92574

Pathways: Signalisation RTK, AMPK Signaling, Regulation of Cell Size, Tube Formation